Measurement
BB123

Part:BBa_K2023001:Design

Designed by: Celia Chenebault, Camille Soucies, Benjamin Piot   Group: iGEM16_Ionis_Paris   (2016-10-14)


Biosensor device for BTEX detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2446
    Illegal NheI site found at 2691
    Illegal NheI site found at 3102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is composed of:

-Pr promoter (constitutive): BBa_I723018

-Elowitz 1999 RBS: BBa_B0034

-XylR condon optimized for E.Coli DH5-Alpha: BBa_K2023005

-A double terminator: BBa_B0014

-Pu promoter: BBa_I723020

-Elowitz 1999 RBS: BBa_B0034

-Gaussia Luciferase codon optimized for E.Coli DH5-Alpha: BBa_K2023009

-A double terminator: BBa_B0014

Source

References