Measurement
BB123
Part:BBa_K2023001:Design
Designed by: Celia Chenebault, Camille Soucies, Benjamin Piot Group: iGEM16_Ionis_Paris (2016-10-14)
Biosensor device for BTEX detection
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2446
Illegal NheI site found at 2691
Illegal NheI site found at 3102 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2101
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This biobrick is composed of:
-Pr promoter (constitutive): BBa_I723018
-Elowitz 1999 RBS: BBa_B0034
-XylR condon optimized for E.Coli DH5-Alpha: BBa_K2023005
-A double terminator: BBa_B0014
-Pu promoter: BBa_I723020
-Elowitz 1999 RBS: BBa_B0034
-Gaussia Luciferase codon optimized for E.Coli DH5-Alpha: BBa_K2023009
-A double terminator: BBa_B0014